Midfielder Billy Mwanza converted a second half penalty to ensure victory for the hosts and also boost their chances as far as a place at the semis is concerned.
It was Ahly’s first defeat at the group phase and it cost them the dream of becoming the first club to reach the last four.
Nkana have now moved to seven points, one behind Ahly. Tunisia’s Etoile du Sahel have five points whilst Sewe Sport are rooted at the bottom on three points. Both play later in the other Group B match in Sousse.
Ahly matched their host squarely in the first half, holding them in all departments of the game. The best chance of the first half fell to Nkana midfield Ronald Kampamba, who volleyed wide from close range.
Ahly brought on fresh limbs including veteran Emad Moteab, Burkinabe midfielder Moussa Yedan and Walid Soliman to boost their search for the equalizer. However, Soliman fumbled with the best chance of the visitors 13 minutes from time but his goal-bound shot was cleared off the line.