It will be the Boks' first visit to the home of English rugby since they played there last November, beating England in a pulsating encounter.
When asked about his return to Twickenham this weekend, Louw, who will earn his 41st cap for South Africa on Saturday, said: "My last match there, for Bath in the Premiership Final, didn't end well, but that is irrelevant now as this is a new team, a new opponent and a new competition.
"The focus for me is now solely on the Springboks and Saturday's quarter-final against a very good Welsh team.
"Twickenham is a fantastic place to play at, in front of a very knowledgeable and vibrant crowd. It's the home of English rugby and the vibe on Saturday will be electric on such a huge occasion.
"We certainly can't wait for the match and although we've recently enjoyed some good results there, that is history - we've got a plan to execute this weekend and we can't wait for the challenge."
Louw said the Springboks will have to brace themselves for a physical onslaught from Wales, with the breakdown a crucial area of the game.
"They are very efficient at the breakdown and will try and get guys to come quick over the ball," said Louw. "The breakdown will be crucial if we want to stop each other's momentum in the game."