They include AS Kigali defender Eric Iradukunda; APR trio, Aimable Nsabimana, Fiston Nkizingabo and Onesme Twizeyimana and Olivier Niyonzima of Rayon Sports.
Midfielder Andrew Buteera also makes a return to the team first time after a one-year hiatus.
The outcome of the encounter will only serve academic purposes with the already qualified Ghanaians leading the pack in Group H with 13 points, seven more than Rwanda.
Amavubi lost the reverse 1-0 in Kigali last September thanks to a Mubarak Wakaso strike late in the second half.
Goalkeepers: Eric Ndayishimiye (Rayon Sports), Jean Claude Ndoli (AS Kigali), Bonheur Hategikimana (SC Kiyovu)
Defenders: Michel Rusheshangoga (APR), Eric Iradukunda (AS Kigali), Emmanuel Imanishimwe (APR), Celestin Ndayishimiye (Police), Herve Rugwiro (APR), Faustin Usengimana (APR), Thierry Manzi (Rayon Sports), Aimable Nsabimana (APR)
Midfielders: Jean Baptiste Mugiraneza (Azam, Tanzania), Yannick Mukunzi (APR), Haruna Niyonzima (Young Africans, Tanzania), Muhadjiri Hakizimana (APR), Andrew Buteera (APR), Djihad Bizimana (APR), Dominique Savio Nshuti (Rayon Sports), Fiston Nkizingabo (APR), Yussuf Habimana (Mukura), Olivier Niyonzima (Rayon Sports), Ally Niyonzima (Mukura)
Forwards: Onesme Twizeyimana (APR), Danny Usengimana (Police), Ernest Sugira (AS Vita, DR Congo), Jacques Tuyisenge (Gor Mahia, Kenya)