The Ghana-born striker joined Zesco in January 2018 from Nchanga Rangers but did not make the cut for the 0-0 first leg draw last week in Zanzibar.
Zikiru got off the mark as early as the third minute when he tapped-in from close-range a Jesse Were lay off.
JKU were on the back foot from then on with Zesco midfielder Enock Sabakumana tormenting the visitors and he tested JKU goalkeeper Haji Ali twice in the 18th and 24th minutes.
Zikiru then comfortably put Zesco 3-0 into the break with a fine shot in the 42nd minute.
The Ghanaian was not done and ensured he took the ball home with two more goals before the hour-mark.
His third came in the 57th minute from an acute angle near the post on the rebound after Haji Ali parried Kondwani Mtonga's header.
Three minutes later, Zikiru wrapped up his historic afternoon at the office and was subsequently substituted in the 70th minute for veteran ex-Zambia striker Winston Kalengo.
Kalengo continued from where Zikiru left off with a close-range shot in the 77th minute to make it 6-0.
His contribution did not end there and three minutes before full-time won a penalty after he was up-ended by Haji Ali.
Zambia youth midfielder Lameck Banda stepped up to convert the penalty to also score his debut goal since joining Zesco this season on loan from South African club Mamelodi Sundowns.
Zesco advance to the pre-group stage 7-0 on aggregate and will be home in the first leg on March 7 to host either ASEC Mimosa of Cote d’Ivoire or Benin club Buffles.
Tuesday, 20 February 2018
Williamsville AC (Cote d’Ivoire) 1-0 Stade Malien (Mali) (1-1)
Olympic de Bangui (Central African Republic) 0-0 ES Setif (Algeria) (0-6)
El Hilal (Sudan) 3-0 LISCR (Liberia) (0-1)
Wednesday, 21 February 2018
KCCA (Uganda) 1-0 CNaPs Sport (Madagascar) (1-2)
Mbabane Swallows (Swaziland) vs Bantu (Lesotho) (4-2)
Aduana (Ghana) 2-0 El Tahadi (Libya) (0-1)
Mounana (Gabon) 2-0 RC Kadiogo (Burkina Faso) (0-1)
MFM (Nigeria) vs AS Real (Mali) (1-1)
MC Alger (Algeria) vs AS Otoho (Congo) (0-2)
Horoya (Guinea) vs AS FAN (Niger) (3-1)
Makkassa (Egypt) vs Generation Foot (Senegal) (0-2)
Saint Louis (Seychelles) 1-1 Young Africans (Tanzania) (0-1)
El Merreikh (Sudan) vs Township Rollers (Botswana) (0-3)
Leones Vegatarianos (Equatorial Guinea) vs Gor Mahia (Kenya) (0-2)
Esperance (Tunisia) vs Concorde (Mauritania) (1-1)
Eding Sport (Cameroon) 0-1 Plateau United (Nigeria) (0-3)
AS Port (Togo) vs AC Leopards (Congo) (1-2)
Zesco (Zambia) 7-0 JKU (Zanzibar) (0-0)
ASEC Mimosas (Cote d’Ivoire) vs Buffles (Benin) (1-1)
UD Songo (Mozambique) 2-0 Ngaya (Comoros) (1-1)
Be Forward (Malawi) vs AS Vita (DR Congo) (0-4)
FC Platinum (Zimbabwe) 1-2 Primeiro de Agosto (Angola) (0-3)
Pamplemousses (Mauritius) 1-0 Bidvest (South Africa) (0-2)
Difaa El Jadida (Morocco) 10-0 Benfica (Guinea Bissau)
Benfica (Guinea Bissau) 0-0 Difaa El Jadida (Morocco) (0-10)
Lydia Academic (Burundi) 0-1 Rayon Sports (Rwanda) (1-1)
Saturday, 24 February 2918
Armed Forces (Gambia) vs Zanaco (Zambia) (0-3)